Fig. 9

Effects of Jawoongo on cytokine expression in splenocytes. Splenocytes were stimulated with LPS (1 μg/ml) and then treated with different concentrations of Jawoongo (25–200 μg/ml) for 24 h. IL-4, IL-6 and TNF-α mRNA expression was measured by RT-PCR (a). The culture medium of the cells was harvested, and IL-6 and TNF-α cytokine levels were measured by ELISA (b). Whole cell lysates were analyzed by Western blotting (c). The data were presented as mean ± SEMs (n = 8 mice/group). *P < 0.05, **P < 0.01 and ***P < 0.001 as compared to LPS-stimulated group, respectively