Fig. 8From: Ethanol extracts from the branch of Taxillus yadoriki parasitic to Neolitsea sericea induces cyclin D1 proteasomal degradation through cyclin D1 nuclear exportCyclin D1 nuclear export contributes to cyclin D1 degradation by TY-NS-B. a HCT116 cells were pretreated with 50 nM of LMB and then co-treated with TY-NS-B (50 μg/ml). b HCT116 cells were transfected with HA-tagged wild type-cyclin D1 or T286A-cyclin D1, and then co-treated with TY-NS-B (50 μg/ml). After treatment, cytoplasm and nucleus were prepared. c HCT116 and SW480 cells were treated with TY-NS-B. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1, HA-cyclin D1 and CRM1. Actin was used as internal control for Western blot analysis. Relative density for Western blot was measured using the software Un-SCAN-IT gel Version 5.1 (Silk Scientific, Inc). Data represent mean ± SD for three independent experiments. *P < 0.05 compared to cell without TY-NS-BBack to article page