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Fig. 8 | BMC Complementary and Alternative Medicine

Fig. 8

From: Ethanol extracts from the branch of Taxillus yadoriki parasitic to Neolitsea sericea induces cyclin D1 proteasomal degradation through cyclin D1 nuclear export

Fig. 8

Cyclin D1 nuclear export contributes to cyclin D1 degradation by TY-NS-B. a HCT116 cells were pretreated with 50 nM of LMB and then co-treated with TY-NS-B (50 μg/ml). b HCT116 cells were transfected with HA-tagged wild type-cyclin D1 or T286A-cyclin D1, and then co-treated with TY-NS-B (50 μg/ml). After treatment, cytoplasm and nucleus were prepared. c HCT116 and SW480 cells were treated with TY-NS-B. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1, HA-cyclin D1 and CRM1. Actin was used as internal control for Western blot analysis. Relative density for Western blot was measured using the software Un-SCAN-IT gel Version 5.1 (Silk Scientific, Inc). Data represent mean ± SD for three independent experiments. *P < 0.05 compared to cell without TY-NS-B

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