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Fig. 7 | BMC Complementary and Alternative Medicine

Fig. 7

From: Ethanol extracts from the branch of Taxillus yadoriki parasitic to Neolitsea sericea induces cyclin D1 proteasomal degradation through cyclin D1 nuclear export

Fig. 7

Determination of factors involved in cyclin D1 degradation by TY-NS-B. a HCT116 cells were pretreated with 20 μM of PD98059, SB203580 or SP600125. b Right panel: HCT116 cells were pretreated with 20 mM of LiCl. Left panel: HCT116 cells were treated with 20 mM of LiCl (c) HCT116 cells were pretreated with 20 μM of LY294002. d HCT116 cells were pretreated with 5 and10 μM of BAY 11–7082. e HCT116 cells were pretreated with 10 mM of NAC. After pretreatment of each inhibitor, HCT116 cells were co-treated with TY-NS-B (50 μg/ml). Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1. Actin was used as internal control for Western blot analysis. Relative density for Western blot was measured using the software Un-SCAN-IT gel Version 5.1 (Silk Scientific, Inc). Data represent mean ± SD for three independent experiments. *P < 0.05 compared to cell without TY-NS-B

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