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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Ethanol extracts from the branch of Taxillus yadoriki parasitic to Neolitsea sericea induces cyclin D1 proteasomal degradation through cyclin D1 nuclear export

Fig. 4

The effect of TY-NS-B on cyclin D1 degradation. a HCT116 and SW480 cells were pretreated with MG132 (10 and 20 μM) and then co-treated with TY-NS-B (50 μg/ml). Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1. Actin was used as internal control for Western blot analysis. Relative density for Western blot was measured using the software Un-SCAN-IT gel Version 5.1 (Silk Scientific, Inc). Data represent mean ± SD for three independent experiments. *P < 0.05 compared to cell without TY-NS-B. b HCT116 and SW480 cells were pretreated with MG132 (20 μM) and then co-treated with TY-NS-B (50 μg/ml) for 24 h. Cell growth was measured using MTT assay. Data represent mean ± SD for three independent experiments. *P < 0.05 compared to cell without TY-NS-B

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