Skip to main content

Advertisement

Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Ethanol extracts from the branch of Taxillus yadoriki parasitic to Neolitsea sericea induces cyclin D1 proteasomal degradation through cyclin D1 nuclear export

Fig. 2

The effect of TY-NS-B on the cell growth and cyclin D1 expression in human colorectal cancer cells. a HCT116 and SW480 cells were plated overnight and then treated with TY-NS-B at the indicated concentrations for 24 h. Cell growth was measured using MTT assay. Data represent mean ± SD for three independent experiments. *P < 0.05 compared to cell without TY-NS-B. b HCT116 and SW480 cells were plated overnight and then treated with TY-NS-B at the indicated concentrations for 24 h. For Western blot analysis, cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1. Actin was used as internal control for Western blot analysis. For RT-PCR analysis of the gene expression of cyclin D1, total RNA was prepared. GAPDH was used as internal control for RP-PCR. Relative density for Western blot and RT-PCR was measured using the software Un-SCAN-IT gel Version 5.1 (Silk Scientific, Inc., Orem, UT, USA). Data represent mean ± SD for three independent experiments. *P < 0.05 compared to cell without TY-NS-B. c HCT116 cells were plated overnight and then treated with TY-NS-B at the indicated concentrations for 24 h. Cell cycle progression was analyzed by flow cytometer

Back to article page