Fig. 6

Choline acetyltransferase (ChAT, red) immunohistochemical studies. a Shows a retina that have undergone the sham procedure (Sham); cell nuclei are counterstained with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI, blue). Amacrine cell bodies (Sham; short arrows) can be seen in the INL and GCL and their neuronal processes (long arrow) display a two-band pattern in the IPL. b, e Show retinas that have undergone I/R together with pre−/post-administration of vehicle (Vehicle+I/R or I/R + Vehicle). A considerable reduction in the IPL immunoreactivity can be seen together with a great reduction in the number of amacrine cell bodies. c, d, f show sectioned retinas that have received I/R and pre-administration of 0.5 g/kg/day (c, DNL0.5 + I/R), or 1.0 g/kg/day of DNL (d, DNL1.0 + I/R), or that have received I/R and post-administration of 1.0 g/kg/day of DNL (f, I/R + DNL1.0). In these groups, the ischemia-induced changes can be seen to be obviously and dose-dependently mitigated when the ischemic retinas have received pre-administration of 0.5 and 1.0 g/Kg/day of DNL. Post-administration of 1.0 g/Kg/day of DNL can also be seen to obviously mitigate these ischemia-induced changes. Abbreviations: ONL, outer nuclear layer, OPL, outer plexiform layer, INL, inner nuclear layer, IPL, inner plexiform layer, GCL, ganglion cell layer. DNL, Dendrobium nobile Lindley. Scale bar = 25 μm