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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Dendrobium nobile Lindley and its bibenzyl component moscatilin are able to protect retinal cells from ischemia/hypoxia by dowregulating placental growth factor and upregulating Norrie disease protein

Fig. 2

The effect of moscatilin on the protein expression levels of NDP relative to β-actin. Top: a series representative immunoblotting images. Bottom: bar chart. The protein expression level of NDP vs. β-actin in the normal control (DMSO+DMEM: RGC-5 cells cultured in DMEM and pre-administration of DMSO) was adjusted to 100%. ** indicates a significant difference (P < 0.01) between the “DMSO+DMEM” group and the “DMSO+OGD” group. † indicates a significant difference (P = 0.048) between the “DMSO+OGD” and “Pre-OGD 0.1 μM Mos” groups (0.1 μM moscatilin given 1 h pre-OGD). The results are presented as means±S.E.M. (n = 3). Please refer to definitions of various groups in Table 1. Abbreviations: NDP, Norrie disease protein; DMSO, dimethyl sulfoxide; DMEM, Dulbecco’s modified Eagle’s medium; OGD, oxygen glucose deprivation; RGC, retinal ganglion cell

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