Fig. 6

Effects of Zyflamend on pAMPK (at Thr172) and pLKB1 (at Ser428) in HeLa cells null for LKB1, transfected with wild type (WT) LKB1 or with two catalytically dead (KD) mutants of LKB1. a Western blot of pAMPK in HeLa cells ± AICAR, (1 mM, 1 h), ionomycin (1 μM, 1 h) or Zyflamend (200 μg/mL, 30 min – 3 h). (b) Western blot of pAMPK in HeLa cells, WT-LKB1 HeLa cells, and KD-LKB1 HeLa cells ± AICAR (1 mM, 1 h), ionomycin (1 μM, 1 h), and/or Zyflamend (200 μg/mL, 1 h). Representative immunoblots from 3 independent experiments are shown. Abbreviations: Con, Control; DMSO, dimethyl sulfoxide; Ion, ionomycin; Zyf, Zyflamend