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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Water/ethanol extract of Cucumis sativus L. fruit attenuates lipopolysaccharide-induced inflammatory response in endothelial cells

Fig. 3

Effect of Cucumis sativus L. extract (CSE) on LPS induced HO-1 expression. a Expression of HO-1 mRNA in pAECs treated with LPS (10 μg/ml) in the presence or absence of increasing doses of Cucumis sativus L. extract (0.02, 0.2, 2 mg/ml). mRNA expression of HO-1 as determined by quantitative PCR. Relative expression was calculated as fold of change in respect to the control cells and error bar represents the range of relative gene expression. Different letters above the bars indicate significant differences. b Representative Western Blot of HO-1 and relative housekeeping α-tubulin were reported. c Expression of HO-1 protein in pAECs treated with LPS (10 μg/ml) in the presence or absence of increasing doses CSE (0.02; 0.2; 2 mg/ml); data shown are representative of at least three independent experiments and represent the mean ± SEM. (AU = Arbitrary Units) Different letters above the bars indicate significant differences (p < 0.05 ANOVA post hoc Tukey’s test)

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