Table 1 The primers specification for amplification of the targeted genes
Gene name | Primer sequence (5′ to 3′) | Accession number | Position | Size (bp) |
|---|---|---|---|---|
PR-NP-F | TCAGTGATTATGAGGGACGGrUTGAT/3Sp | CY148246.1 | 179–198 | 97 |
PR-NP-R | TTCTTCCAGGTATTTATTTCTCCTrUTCGTT/3Sp | 253–276 | ||
PR-M2-F | GCAGTTAAACTGTATAGGAAGCTrCAAGA/3Sp | CY148244.1 | 311–333 | 69 |
PR-M2-R | CACCAGCAGAATAACTGAGTGrAGATTC/3Sp | 360–380 | ||
TNF-α-F | ATCAATCTGCCTAACTATCT | NM_001003244.4 | 634–653 | 168 |
TNF-α-R | CTGAGCCCTTAATTCTCT | 785–802 | ||
IL-27-F | GCTGTTCTCAGAGGTTCGG | XM_844736.3 | 258–276 | 75 |
IL-27-R | CAGGAGGTCCAGGCTTACT | 315–333 | ||
GusB-F | TGCTCCTCTACACCACACCTAC | NM_001003191.1 | 532–553 | 80 |
GusB-R | CCACCAGCCCAGTGTCTTG | 594–612 | ||
ACTB-F | CAGGAGTACGACGAGTCCG | NM_001195845.1 | 1209–1227 | 87 |
ACTB-R | CAAGAAAGGGTGTAACGCAACT | 1275–1296 |