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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Homoharringtonine regulates the alternative splicing of Bcl-x and caspase 9 through a protein phosphatase 1-dependent mechanism

Fig. 6

Overexpression of PP1 increases alternative splicing and promotes tumor cell apoptosis induced by HHT. A stable MCF7 cell line overexpressing PP1 was constructed with a lentiviral expression system. MCF7-Bobi and MCF7-PP1 cells were treated with 0.5 μM HHT. Total RNA was extracted and analyzed by semi-quantitative RT-PCR for the splicing of Bcl-x and Caspase 9. a Overexpression of PP1 in MCF7 cells. b Semi-quantitative RT-PCR analysis of Bcl-x and Caspase 9 splicing from MCF7-Bobi and MCF7-PP1 cells treated with 0.5 μM HHT. c and d Ratio of Bcl-xL/xS and Caspase 9a/9b in MCF7-Bobi and MCF7-PP1 cells (*P < 0.05). e Apoptosis induced by 5 μM HHT in MCF7-Bobi and MCF7-PP1 cells (*P < 0.05)

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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