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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Homoharringtonine regulates the alternative splicing of Bcl-x and caspase 9 through a protein phosphatase 1-dependent mechanism

Fig. 3

HHT regulates Bcl-x and Caspase 9 splicing in A549 and UACC903 cells. Total RNA was extracted from A549 cells and UACC903 cells treated with HHT. The alternative splicing of Bcl-x and Caspase 9 was then analyzed by semi-quantitative RT-PCR. a Semi-quantitative RT-PCR analysis of Bcl-x splicing from A549 cells treated with different concentrations of HHT. b Densitometric analysis of the ratio of Bcl-xL/xS in A549 cells treated by HHT (*P < 0.05 compared to cells treated with control). c Semi-quantitative RT-PCR analysis of Caspase 9 splicing from A549 cells treated with different concentrations of HHT. d Densitometric analysis of the ratio of Caspase 9a/9b in A549 cells treated by HHT. e Semi-quantitative RT-PCR analysis of Bcl-x splicing from UACC903 cells treated with different concentrations of HHT. (f) Densitometric analysis of the ratio of Bcl-xL/xS in UACC903 cells treated by HHT (*P < 0.05 compared to cells treated with control). g Semi-quantitative RT-PCR analysis of Caspase 9 splicing from UACC903 cells treated with different concentrations of HHT. h Densitometric analysis of the ratio of Caspase 9a/9b in UACC903 cells treated by HHT (*P < 0.05 compared to cells treated with control)

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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