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Table 1 Primers sequences of target genes

From: Curcumin derivative, 2,6-bis(2-fluorobenzylidene)cyclohexanone (MS65) inhibits interleukin-6 production through suppression of NF-κB and MAPK pathways in histamine-induced human keratinocytes cell (HaCaT)

Genes   Primer sequences (5′-3′) Accession number
H1R F R CTG GTT TCT CTC TTT TCT GTG GGT T GAT CTT GGC ATA GAA CCA GAG CAT G NM_001098213.1
PKC F R CAG GCA GAA ATT CGA GAA AGC CAA A TCC CAA CAC CAT GAG GAA ATT GAA G NM_002737.2
IKK-β F R ATC CCC GAT AAG CCT GCC A CTT GGG CTC TTG AAG GAT ACA G NM_001556.2
IκB-α F R ATT GCT GAG GCA CTT CTG GGA GCT G AGA CAC GTG TGG CCA TTG TAG TTG G NM_020529.2
NF-κβ F R GGA CCG CTG CAT CCA CAG TTT CCA G TGT CAC CTG GAA GCA GAG CCG CAC A NM_001145138.1
c-Raf F R CAG TAT CTG GGA CCC AGG AGA AAA A TAG GAT CTT TAC TGC AAC ATC TCC G NM_002880.3
MEK F R CGT ACA TCG TGG GCT TCT ATG GTG C GCA TGA TCT TGT GCT TCT CCC TCA G NM_002755.3
ERK F R CTC TAC CAG ATC CTC AGA GGG TTA A TAC CAA CGT GTG GCC ACA TAT TCT G NM_002745.4
GAPDH F R CAG CCT CAA GAT CAT CAG CA CAT CCA CAG TCT TCT GGG TG NM_002046.5
  1. H1R histamine H1-receptor, PKC protein kinase, IKK-β inhibitor of nuclear factor kappa-B kinase subunit beta, IκB-α Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NF-κβ nuclear factor kappa-light-chain-enhancer of activated B cells, c-Raf RAF proto-oncogene serine/threonine-protein kinase, MEK mitogen-activated protein kinase kinase, ERK extracellular signal–regulated kinases, GAPDH glyceraldehyde 3-phosphate dehydrogenase