Fig. 5From: Puerarin inhibits vascular smooth muscle cells proliferation induced by fine particulate matter via suppressing of the p38 MAPK signaling pathwayEffect of puerarin on the levels of inflammatory and oxidative stress biomarkers in VSMCs. Cells were pre-treated with puerarin at different concentrations (0, 12.5, 25, 50 μM) or p38 MAPK inhibitor SB203580 (20 μM) for 1 h and followed by the addition of 200 mg/L PM2.5 for 24 h. a IL-6 level was measured by ELISA. b TNF-α level was measured by ELISA. c SOD level was assayed by colorimetric assay kit. d MDA level was assayed by colorimetric assay kit. The results are presented as mean ± SEM. n = 3. Compared to the untreated cells, *P < 0.05, **P < 0.01; compared to 200 mg/L PM2.5 group, #P < 0.05, ##P < 0.01; compared to the co-incubation of puerarin with SB203580 group, ▲P < 0.05, ▲▲P < 0.01Back to article page