Fig. 4
Bioactivity comparison results of SO, SP and SG. a Effects of water extracts on NO production. b Effects of 50% ethanol extracts on NO production. c Effects of 95% ethanol extracts on NO production. RAW 264.7 cells were treated with various concentrations of individual extract for 1 h and then stimulated with 100 ng/mL LPS for 24 h. NO production was indicated by the level of nitrite in the supernatant measured with the Griess reagent. Inhibition rate (%) of an extract was calculated against LPS alone-treated cells. EC50 was calculated using the curve-fitting software GraphPad Prism 5.0. d Summary of EC50 values shown in (a), (b) and (c). Data are shown as mean ± SD from tree independent experiments. #p < 0.05, *p < 0.01