Fig. 4
The effect of AC on the mRNA expression of skp2 and RhoA and the protein expression of PARP and skp2. a-d Total mRNA was extracted from the two breast cancer cells after treat without or with indicated drugs for 24 h. The coding regions of human Skp2 and RhoA were used as probes for real time polymerase chain reaction analysis. e, f Total cell extracts of the two breast cancer cells were harvested from cells treated with DMSO or indicated concentrations of AC for 24 h. The protein was immunoblotted with polyclonal antibodies specific for PARP or Skp2. β-actin was used as an internal loading control. (Error bars = mean ± S.E.M. Asterisks (*) mark samples significantly different from DMSO group with p < 0.05)