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Table 1 Primer sequences of inducible nitric oxide synthase (iNOS) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) used in the quantitative real time PCR (qRT-PCR) assay. Beta-actin (β-actin), hypoxanthine phosphoribosyltransferase (HPRT) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as housekeeping genes for normalization of the iNOS and NF-kB gene expression

From: Coconut water vinegar ameliorates recovery of acetaminophen induced liver damage in mice

  Forward primer (5′-3′) Reverse primer (3′-5′)
iNOS 5′-GCACCGAGATTGGAGTTC-3′ 3′-GAGCACAGCCACATTGAT-5′
NF-κB 5′-CATTCTGACCTTGCCTATCT-3′ 3′-CTGCTGTTCTGTCCATTCT-5′
β-actin 5′-TTCCAGCCTTCCTTCTTG-3′ 3′-GGAGCCAGAGCAGTAATC-5′
GAPDH 5′-GAAGGTGGTGAAGCAGGCATC-3′ 3′-GAAGGTGGAAGAGTGGGAGTT-5′
HPRT 5′-CGTGATTAGCGATGATGAAC-3′ 3′-AATGTAATCCAGCAGGTCAG-5′