Fig. 4

Effects of PEE on the number of macrophages and MDSCs in the peritoneum of HFD- and normal chow-fed mice. C57BL/6 mice were fed with (a, b) HFD or (c, d) normal chow. PEE (100 mg/kg, twice weekly) or vehicle was intraperitoneally injected into the mice for one month. Cells in the peritoneal cavity were harvested and subjected to FACS analysis for macrophages and MDSCs. a, c Representative FACS images of four replicates. Only F4/80⁺ living cells are shown. b, d Proportion of macrophages and MDSCs in F4/80⁺ myeloid cells in the peritoneum of (b) HFD-fed and (d) normal chow-fed mice. Data represents means ± SD of four samples. *P < 0.05 vs. vehicle-treated mice