Table 2. Characteristics of sequences and derived designed primers for high resolution melting analysis

Available species

199

271

142

156

Number of species in analysed data set

178

256

136

98

Characters in an in silico analysis (bp)

158

181

118

315

Variable characters (%)

135/158 (85.44%)

42/181 (23.20%)

36/118 (30.51%)

274/315 (86.98%)

Conserved sites (%)

23/158 (14.56%)

107/181 (59.12%)

73/118 (61.86%)

41/315 (13.02%)

Average %GC content

34.6

43.4

42.5

53.9