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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Fucoxanthin, the constituent of Laminaria japonica, triggers AMPK-mediated cytoprotection and autophagy in hepatocytes under oxidative stress

Fig. 2

Fucoxanthin (FX) ameliorates AA + iron-induced cytotoxicity. HepG2 cells were incubated with indicated dose of FX for 1 h and later treated with 10 μM AA for 12 h, being followed by exposure to 5 μM iron for 3 h. (a) The chemical structure of FX. (b) The effects of FX on cell viability was assessed by the MTT assay. (c) Expression of proteins associated with apoptosis was determined by western blot analysis. Equal protein loading was verified by β-actin. (d) ΔΨm depolarization monitored by FACS analysis of Rh123 staining. Relative proportions of low Rh-123 intensity (RN1 fractions) are expressed as the mean ± S.D. of three separated experiments. For panel b, c and d, data represent the mean ± S.D. for the four replicates. ** p < 0.01

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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