Fig. 5

Effect of 6-week treatment with Allium fistulosum aqueous extract (AFW) and A. fistulosum ethanolic extract (AFE) on histology and gene expression in the liver tissue of high-fat diet (HFD)-induced obese mice. a Representative photographs of hematoxylin and eosin-stained liver sections (magnification, 400×). b Expression of adenosine monophosphate-activated protein kinase (AMPK) isoforms AMPKα1 and AMPKα2 mRNA in the liver, as assessed by real-time reverse-transcription polymerase chain reaction (RT-PCR). Normal, standard chow; HFD-control, HFD only; HFD-HCA, HFD plus 100 mg/kg Garcinia cambogia extract containing hydroxycitric acid; HFD-CLA, HFD plus 100 mg/kg conjugated linoleic acid; HFD-ORL, HFD plus 15.6 mg/kg Xenical (orlistat); HFD-AFW, HFD plus 100 mg/kg AFW; HFD-AFE, HFD plus 100 mg/kg AFE. Results are expressed as the mean ± standard error of the mean (SEM) (n = 6). * p < 0.05, *** p < 0.001 compared with the HFD-control group