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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: A Chinese herbal decoction, Jian-Pi-Yi-Shen, regulates the expressions of erythropoietin and pro-inflammatory cytokines in cultured cells

Fig. 1

JPYS stimulates the mRNA expressions of EPO and HIF-1α. a Cultured HEK293T cells (5 × 104 cells/ml) were applied with JPYS extracts (1–30 μg/mL) for 48 h. The level of EPO mRNA was detected by real-time PCR. b Cultured HEK293T cells were treated with JPYS extracts as in (a).The level of HIF-1α mRNA was detected by real-time PCR. GAPDH was used as an internal control for normalization. The mineral oil layering (oil: medium = 2: 1) served as a positive control, with the response about 80% and 150% of increase for expressions of EPO and HIF-1α mRNAs, respectively. Values are expressed as % of increase to basal reading (untreated culture), and in Mean ± SD, where n = 3, each with triplicate samples. Statistical comparison was made with the control; * p < 0.05; ** p < 0.01

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