Treatment | Number of Colonies | Size of Colonies (mm) |
---|
DMSO | 41 | 0.8–1.2 |
AA (20 nM) | 32 | 0.8–1.2 |
5-FU (5 nM) | 10 | 0.1–0.2 |
5-FU/AA | 5 | 0.1–0.2 |
GEM (5 nM) | 7 | 0.1–0.2 |
GEM/AA | 5 | 0.1–0.2 |
- The same number of cells were seeded onto Nunclonâ„¢ Sphera plates. Next day, media was replaced with new media containing the following reagents as described in the table. Every other day, media was replaced with fresh media containing respective reagent(s). 7Â days after the initial treatment, the number of spheroids was counted, and the size of spheroids measured in diameter. The data was collected from two independent assays