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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Repeated electroacupuncture treatment attenuated hyperalgesia through suppression of spinal glial activation in chronic neuropathic pain rats

Fig. 2

Numbers of Iba-1-labeled microgliacytes in ipsilateral and contralateral dorsal horns (DHs) of lumbar spinal cord (L4–5) on day 6 and 18 after surgery in the normal control (normal), sham operation (sham), and CCI model (CCI) groups. a Iba-1-labeled microglia (indicated by arrowheads) in the DHs and anterior horns of lumbar spinal cord on the ipsi. Side of CCI on day 6 after surgery. b An increase of numbers of the activated microgliacytes mainly distributing in the superficial layers of ipsi. and cont. DHs on day 6 (denser) and day 18 (lesser) in the 3 groups. Central column: the magnified activated microgliacytes derived from the dashed rectangular box of each picture on the left side. Ipsi: ipsilateral, Cont: contralateral. D: day. c Histograms showing the number of Iba-1-labeled microgliacytes in the ipsilateral and contralateral DHs of lumbar spinal cord on day 6 and 18 after sham and CCI-operation in the 3 groups (mean ± SD, n = 6/group). ^ P < 0.05, ^^ P < 0.01, vs the normal group; # P < 0.05, ## P < 0.01, vs the sham group

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