Fig. 7

Effect of GRC-ON89A and β-glucan on macrophage activity and morphology. a Cell morphological changes of β-glucan or GRC-ON89A treated RAW 264.7 cells. RAW 264.7 cells were treated with GRC-ON89A, β-glucan (0.1, 1, 10, 100 μg/ml) or LPS (1 μg/ml). Arrows mediated RAW264.7 cells with increased cellular size. b The cell morphology of RAW 264.7 by GRC-ON89A, β-glucan (100 μg/ml) for 0, 24, 48, and 72 h was observed using Metamorph software for real time imaging (magnification, 200×). Arrows mediated RAW 264.7 cells with increased cellular size. c The level of NO production in RAW 264.7 cells by β-glucan. One-way ANOVA was used for comparison of group means, followed by Dunnett’s t-test (^# = p < 0.005 vs. control group). Each figure is representative of three independent experiments; Scale bars = 100 μm