Fig. 4

Effects of GRC-ON89A on the phagocytic activity of peritoneal macrophages in normal mice. a Scheme summarizing the normal murine model protocol. b Fluorescent microscopy images (left panel) of phagocytosis of primary peritoneal macrophages from CY-treated immunosuppressed model group after oral administration (D.W., 1% GRC and 1% GRC-ON89A). Quantification of fluorescent bead uptake by primary peritoneal macrophages from CY-treated immunosuppressed groups exposure to fluorescent beads for 48 h (right panel). The phagocytic index was quantified for statistics analysis using Image J software. One-way ANOVA was used for comparison of group means, followed by Dunnett’s t-test (** = p < 0.01 vs GRC). Each figure is representative of three independent experiments; Scale bars = 50 μm