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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Citral induced apoptosis in MDA-MB-231 spheroid cells

Fig. 6

a Inhibitory effect of citral on ALDH positive-cell population. The MDA-MB-231 spheroids were treated with citral (2.5, 5 and 10 μg/mL) and subject to Aldefluor assay flow cytometry analysis. Citral decreased the percentage of ALDH-positive cells. b Relative expression level of APC, AXIN, CK1 and LRP6 in vehicle control and citral treated MDA-MB-231 spheroids. c Representative blot of β-catenin, phospho β-catenin and cycline D1 protein level in vehicle control and citral treated MDA-MB-231 spheroids. d Quantification of western blot analysis for β-catenin, phospho β-catenin and cycline D1 protein level in vehicle control and citral treated MDA-MB-231 spheroid using ImageJ software. All the experiments were performed in three biological replicates and each with triplicates. All data are expressed as mean ± S.E.M. with (*p < 0.05)

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