Fig. 5

Annexin V/7AAD flowcytometry. MDA-MB-231 spheroids with low concentration (2.5 μg/mL) of citral, showed 8.99 ± 2.20% and 9.78 ± 2.10% for early and late apoptosis respectively, increasing the concentration to 5.0 μg/mL showed 38.41 ± 3.34% and 31.24 ± 3.14% and the high concentration (10.0 μg/mL) showed 43.28 ± 3.46% and 38.67 ± 2.77% for early and late apoptosis respectively compared to vehicle control that showed 1.19 ± 0.74% and 1.44 ± 0.82% of early and late apoptosis. All the experiments were performed in three biological replicates and each with triplicates. All data are expressed as mean ± S.E.M. with (*p < 0.05)