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Table 2 Results of the in vitro chromosomal aberration test for SOCG

From: Genotoxicity evaluation of So-ochim-tang-gamibang (SOCG), a herbal medicine

Dose (μg/mL) S9 mix Trt-Rec Timed (hrs) Proliferation rate (%) Structural aberrations (%)e Numerical aberration (%)g
ctb cte csb cse total end pol total
Short duration treatment without metabolic activation
 Negative controla 6–18 100 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
 288 6–18 85.3 0.0 0.0 0.0 0.0 0.0 0.0 0.5 0.0
 575 6–18 88.1 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
 1150 6–18 76.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
 2300 6–18 29.9 Toxic
 Positive controlb 6–18   7.0 14.0 0.0 0.0 17.5* 0.0 0.0 0.0
Short duration treatment with metabolic activation
 Negative controla + 6–18 100 0.5 0.0 0.0 0.0 0.5 0.0 0.0 0.0
 438 + 6–18 87.8 0.0 0.0 0.0 0.0 0.0 0.0 0.5 0.5
 875 + 6–18 87.8 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
 1750 + 6–18 70.7 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
 3500 + 6–18 57.7 Toxic
 Positive controlc + 6–18   7.5 0.0 0.0 19.0 23.0* 0.0 0.5 0.5
Continuous treatment without metabolic activation
 Negative controla 24–0 100 0.5 0.0 0.0 0.0 0.5 0.0 0.0 0.0
 88.8 24–0 113 not observed
 178 24–0 76.2 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
 355 24–0 71.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
 710 24–0 58.7 0.0 0.5 0.0 0.0 0.5 0.0 0.0 0.0
 Positive controlb 24–0   5.0 26.5 0.0 0.0 30.0* 0.0 0.0 0.0
  1. aWater for injection
  2. bMitomycin C, 0.1 μg/mL, c Benzo[α]pyrene, 20 μg/mL, d Trt-Rec Time: Treatment-Recovery times
  3. eStructural aberration (%): The number of cells with structural chromosome aberration in metaphase cells. Ctb: chromatid breakage; cte: chromatid exchange; csb: chromosome breakage; cse: chromosome exchange; total: total number of cells with structural chromosome aberration except for gap in metaphase cells
  4. gNumerical aberration (%): The number of cells with numerical chromosome aberrations in metaphase cells; end: endoreduplication; pol: polyploidy; total: total number of cells with numerical chromosome aberrations in metaphase cells
  5. Significant difference from negative control by fisher’s exact test: * p < 0.01