Genotoxicity evaluation of So-ochim-tang-gamibang (SOCG), a herbal medicine

Table 2 Results of the in vitro chromosomal aberration test for SOCG

Dose (μg/mL)	S9 mix	Trt-Rec Time^d (hrs)	Proliferation rate (%)	Structural aberrations (%)^e					Numerical aberration (%)^g
Dose (μg/mL)	S9 mix	Trt-Rec Time^d (hrs)	Proliferation rate (%)	ctb	cte	csb	cse	total	end	pol	total
Short duration treatment without metabolic activation
Negative control^a	–	6–18	100	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
288	–	6–18	85.3	0.0	0.0	0.0	0.0	0.0	0.0	0.5	0.0
575	–	6–18	88.1	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
1150	–	6–18	76.3	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2300	–	6–18	29.9	Toxic
Positive control^b	–	6–18		7.0	14.0	0.0	0.0	17.5*	0.0	0.0	0.0
Short duration treatment with metabolic activation
Negative control^a	+	6–18	100	0.5	0.0	0.0	0.0	0.5	0.0	0.0	0.0
438	+	6–18	87.8	0.0	0.0	0.0	0.0	0.0	0.0	0.5	0.5
875	+	6–18	87.8	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
1750	+	6–18	70.7	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
3500	+	6–18	57.7	Toxic
Positive control^c	+	6–18		7.5	0.0	0.0	19.0	23.0*	0.0	0.5	0.5
Continuous treatment without metabolic activation
Negative control^a	–	24–0	100	0.5	0.0	0.0	0.0	0.5	0.0	0.0	0.0
88.8	–	24–0	113	not observed
178	–	24–0	76.2	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
355	–	24–0	71.4	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
710	–	24–0	58.7	0.0	0.5	0.0	0.0	0.5	0.0	0.0	0.0
Positive control^b	–	24–0		5.0	26.5	0.0	0.0	30.0*	0.0	0.0	0.0

^aWater for injection
^bMitomycin C, 0.1 μg/mL, ^c Benzo[α]pyrene, 20 μg/mL, ^d Trt-Rec Time: Treatment-Recovery times
^eStructural aberration (%): The number of cells with structural chromosome aberration in metaphase cells. Ctb: chromatid breakage; cte: chromatid exchange; csb: chromosome breakage; cse: chromosome exchange; total: total number of cells with structural chromosome aberration except for gap in metaphase cells
^gNumerical aberration (%): The number of cells with numerical chromosome aberrations in metaphase cells; end: endoreduplication; pol: polyploidy; total: total number of cells with numerical chromosome aberrations in metaphase cells
Significant difference from negative control by fisher’s exact test: * p < 0.01

ISSN: 2662-7671