Fig. 1

The effect of TC-HW on the cell viability and cyclin D1 expression in human colorectal cancer cells. a HCT116, SW480, LoVo and HT-29 cells were plated overnight and then treated with TC-HW at the indicated concentrations for 24 h. Cell viability was measured using MTT assay. *P < 0.05 compared to cell without TC-HW. b-e HCT116, SW480, LoVo and HT-29 cells were plated overnight and then treated with TC-HW at the indicated concentrations for 24 h. For Western blot analysis (b, d), cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1. Actin was used as internal control for Western blot analysis. For RT-PCR analysis of the gene expression of cyclin D1 (c, e), total RNA was prepared. GAPDH was used as internal control for RP-PCR. f HCT116 cells were plated overnight and then treated with TC-HW at the indicated concentrations for 24 h. Cell cycle progression was analyzed by flow cytometer. g HCT116 and SW480 cells were pretreated with MG132 (10 μM), and then co-treated with TC-HW (100 μg/ml). Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1. Actin was used as internal control for Western blot analysis. *P < 0.05 compared to cell without TC-HW