Fig. 3

NVH HPLC, TLC and HR-LC-MS profiles. a: NVH HPLC profile using acetonitrile at different percentages and time ranges (0, 0, 5, 5, 10, 20, 100% respectively during 1–10, 10–15, 15–20, 20–25, 25–35, 35–50, 50–60 min). The upper part represents NVH HPLC profile at 366 nm and the lower part, NVH HPLC profile at 254 nm. b: NVH TLC fingerprint at 254 nm (left) and 366 nm (right) using water: methanol: acetic acid (12.5,12.5:1). c: NVH HR-LC-MS profile with the four identified amino acids