Fig. 12

In vitro extract antioxidant capacity and ferric reducing antioxidant power (FRAP). a: graph showing total antioxidant capacity of the extract (triangle) compared to vitamin C (circle) and silymarin (square). Results are expressed as optical density mean ± Standard Error of the Mean. b: graph showing the ferric reducing capacity of the extract (triangle) compared to vitamin C (circle) and BHT (square). Results are expressed as optical density mean. c: DPPH scavenging capacity of the extract. In 3 different experiments, the extract (triangle) DPPH inhibition kinetic was assay within 120 min. Vitamin C (circle) and silymarin (square) were used as references. (d-e): DPPH inhibition by TLC method. The DPPH scavenging capacities of the extract and its main fractions were visualized on silica plates after elution in water: methanol: acetic acid (12.5/12.5/1). Yellow points represent the scavenging capacity of the extract and its main fractions and compound. One and 2 respectively represent the contact points of the extract and its main fractions with DPPH while 3 and 4 respectively represent the contact points of F3 fraction and F3_52–53 compound with DPPH. DPPH: 1,1-diphenyl-2-picrylhydrazyl; NVH: Neoboutonia velutina aqueous extract; TLC: thin layer chromatography; BHT: Butylated Hydroxytoluene