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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Combination of Pelargonium sidoides and Coptis chinensis root inhibits nuclear factor kappa B-mediated inflammatory response in vitro and in vivo

Fig. 2

Inhibition of nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression by PS + CR. RAW 264.7 cells were treated with 3–300 μg/ml PS + CR for 1 h prior to the addition of lipopolysaccharide (LPS) (1 μg/ml) and further incubated for 24 h. a Effects of PS + CR on NO production. Secreted nitrite concentrations in the culture medium were assayed using the Griess reagent method. b Effects of PS + CR on iNOS protein expression. Equal amounts of total protein (50 μg) were separated by SDS-PAGE. β-Actin was used as a loading control, and the bar chart shows the quantitative evaluation of iNOS bands by densitometry. (c) Effects of PS + CR plus LPS on cell viability. Cell viability was measured by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay after 24 h of incubation. Values represent the mean ± S.D. of three independent experiments (significant compared to the control, **p < 0.01; significant compared to LPS alone, #p < 0.05 or ##p < 0.01)

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