Fig. 2
From: Shuang-Huang-Lian prevents basophilic granulocyte activation to suppress Th2 immunity
Effect of SHL on the cytokines produced by splenocytes. The mice were intraperitoneally immunized with ST (60 μg/mouse) once a week. SHL (3 mL/kg or 6 mL/kg, i.g.) was administered daily to the mice. 4 weeks later the spleens were taken, and single cell suspensions were aseptically prepared. The obtained splenocytes (4 × 106 cells/well) were seeded in a 24-well plate and stimulated with ST (8 μg/mL) at 37 °C for 72 h. The levels of IL-4 (a), IL-5 (b), IL-10 (c), IL-13 (d) and IFN-γ (e), in the culture medium, were measured using the ELISA kits. The data represent the means ± SD (n = 10). ##P < 0.01 vs. the normal control group; *P < 0.05 vs. the ST group