Fig. 3

BP induced mitochondrial-mediated apoptosis in human bladder cancer cells. a Human bladder cancer cells were treated with 0.2% DMSO (control) or 60 μg/ml BP for 72 h and then stained with the TUNEL assay. TUNEL positive cells (green fluorescence) were indicated by arrows. Scale bar: 50 μm. b Human bladder cancer cells were treated with 60 μg/ml BP for 0 to 72 h, and western blot analysis were performed for cleaved caspase-9 and -3. β-actin was used as an internal control. c MTT assay of human bladder cancer cells pretreated with caspase 3 inhibitor Z-DEVD-fmk (20 μM) for 1 h and then treated in the presence or absence of 60 μg/ml BP for 48 h. The values are the mean ± S.D. from three independent experiments, * p < 0.05 versus vehicle