Gene | Realtime RT-PCR | ||
---|---|---|---|
24 h | 36 h | 48 h | |
ER stress | |||
GRP78 | 1.02 ± 0.16 | 10.82 ± 3.05** | 10.06 ± 3.05** |
ERN1 | 1.68 ± 0.32 | 8.86 ± 6.54* | 2.10 ± 0.53* |
ATF4 | 1.75 ± 0.37* | 3.67 ± 0.61** | 1.11 ± 0.24 |
XBP1 | 2.71 ± 1.84 | 2.17 ± 0.06** | 0.60 ± 0.24 |
DDIT3 | 0.94 ± 0.59 | 26.45 ± 14.47* | 4.56 ± 1.57* |
FAM129A | 4.52 ± 1.84* | 44.20 ± 11.70** | 34.84 ± 9.14** |
GDF15 | 5.65 ± 0.72** | 35.36 ± 9.23* | 19.49 ± 7.78** |
p53 pathway | |||
TP53 | 2.06 ± 0.95 | 2.46 ± 0.05*** | 1.30 ± 0.23 |
RELA | 0.90 ± 0.32 | 1.65 ± 0.26* | 1.14 ± 0.41 |
GADD45A | 2.84 ± 1.17 | 12.82 ± 3.45** | 6.15 ± 3.43* |
CDKN1A | 0.76 ± 0.46 | 3.26 ± 0.17*** | 2.91 ± 0.69* |
Cell cycle | |||
CDK1 | 0.88 ± 0.12 | 0.61 ± 0.07* | 0.41 ± 0.08* |
CDK2 | 0.75 ± 0.29 | 0.64 ± 0.08* | 0.37 ± 0.18 |
CCNB | 0.95 ± 0.33 | 0.71 ± 0.38 | 0.19 ± 0.12* |
CCNE | 0.70 ± 0.32 | 1.01 ± 0.26 | 0.48 ± 0.13* |
CCNA2 | 0.66 ± 0.33 | 0.81 ± 0.17 | 0.50 ± 0.24* |
Apoptosis | |||
BCL2 | 0.37 ± 0.12* | 0.48 ± 0.21 | 0.25 ± 0.11* |
BAX | 0.70 ± 0.23 | 1.02 ± 0.17 | 3.28 ± 1.03* |
CAS9 | 1.30 ± 0.17 | 1.99 ± 0.87 | 2.23 ± 0.44* |
CAS8 | 0.96 ± 0.21 | 2.52 ± 0.62* | 2.30 ± 0.14* |
NOXA | 1.29 ± 0.59 | 4.43 ± 1.75* | 0.57 ± 0.40 |
PUMA | 3.31 ± 3.10 | 6.76 ± 1.75** | 11.95 ± 5.74* |
TRAIL | 2.44 ± 1.87 | 2.44 ± 1.82 | 1.78 ± 0.03*** |
Oxidative stress response | |||
NFE2L2 | 0.91 ± 0.51 | 1.53 ± 0.13* | 1.05 ± 0.61 |
HMOX1 | 5.69 ± 0.90** | 9.37 ± 3.96* | 17.40 ± 7.54* |
CAT | 1.41 ± 0.33 | 1.56 ± 0.31 | 0.64 ± 0.31 |
SOD1 | 1.59 ± 0.43 | 2.44 ± 0.95 | 0.50 ± 0.23 |
SOD2 | 1.10 ± 0.37 | 1.30 ± 0.21 | 0.85 ± 0.05* |
GPX-1 | 1.87 ± 0.31* | 3.83 ± 2.50 | 0.80 ± 0.57 |
Estrogen receptor | |||
ESR1 | 0.81 ± 0.06* | 0.73 ± 0.11 | 0.70 ± 0.29 |
ESR2 | 2.08 ± 1.20 | 2.70 ± 0.61* | 2.03 ± 0.58 |