Fig. 3

Colla corii asini ethanol extract inhibited the proteins and mRNAs of T3SS-1 effectors in S. typhimurium. We detected the secretion of T3SS-1 effectors in the bacterial supernatant by western blotting. a The Colla corii asini ethanol extract (0.195 mg/ml) significantly reduced the salmonella invasion proteins such as SipA, SipB and SipC. b We treated S. typhimurium with various concentrations of Colla corii asini ethanol extract. After 24 h, we extracted mRNA from S. typhimurium and conducted real-time PCR. Colla corii asini ethanol extract (0.195 mg/ml) significantly decreased the mRNA levels of salmonella invasion proteins