Table 1 Oligonucleotide primer sets and conditions used for semi-quantitative RT-PCR
From: Naringenin attenuates hepatitis B virus X protein-induced hepatic steatosis
Primer-name | Sequence |
|---|---|
PPARyl-Forward | 5′-TCTCCATGACAGACATGGACA-3’ |
PPARyl-Reverse | 5′-GTCAGG CTGTTG GTCTCACA-3’ |
PPARy2-Forward | 5′-GGGTGAAACTCTGGGAGATTCTC-3’ |
PPARy2-Reverse | 5′-TCAGCAACCATTGGGTCAG-3’ |
PPARa-Forward | 5′-GCAGCTCGTACAGGTCATCA-3’ |
PPARa-Reverse | 5′-ACTGCCGTT GT CT GT CACT G-3’ |
SREBP-lc-Forward | 5′-TTGTGGAGCTCAAAGACCTG-3’ |
SREBP-lc-Reverse | 5′-TGCAAG AAGCGG AT GT AGTC-3’ |
LXRa-Forward | 5′-TCCTACACGAGGATCAAGCG-3’ |
LXRa-Reverse | 5′-AGTCGCAATGCAAAGACCTG-3’ |
GAPDH-Forward | 5′-AGAACATCATCCCTG CAT CC-3’ |
GAPDH-Reverse | 5′-CACATTGGGGGTAGGAACAC-3’ |
HBx-Forward | 5′-TgTgCT gCCAACTggAT CCTg-3’ |
HBx-Reverse | 5′-CCAATTTATgCCTACAgCCTCC-3’ |