Fig. 5

Effect of GR or ZJW treatment on the Wnt/β-catenin signaling pathway. a SW403 cells were treated with GR (1, 5, and 10 μmol/L, respectively) for 24 h. Representative western blots of Axin1, Dvl2, Dvl3, GSK-3β, LEF1, and TCF4 were shown. b Semi-quantification data of the Western blot relative to β-actin. c Western blotting of β-catenin in total cell lysate and nucleus in SW403 cells treated as (a). d The corresponding semi-quantification data of the Western blot relative to histone 2A. e SW403 cells were treated with ZJW-L, ZJW-M and ZJW-H for 24 h. Representative western blots for Axin1, Dvl2, Dvl3, GSK-3β, serine-phosphorylated-GSK-3β, LEF1 and TCF4 in SW403 cells were shown. f The semi-quantification data of the Western blot relative to β-actin. All values are shown as mean ± SD of three separate experiments, and significant values are indicated with asterisks (*P < 0.05, **P < 0.01 vs. control)