Fig. 1

Effect of the ZJW extracts treatment on 5-HTR1D expression in SW403 cell. SW403 cell was stimulated with GR (1, 5, 10 μmol/L) and ZJW at low, medium, and high dose, respectively. Cells were cultivated for 24 h. Protein expression in cells with different stimulation was determined by Western blot. Results were normalized to β-actin expression. a Representative western blots of 5-HTR1D protein in GR-treated SW403 cells. b The corresponding semi-quantification data of (a). c Representative western blots of 5-HTR1D protein in ZJW-treated and GR (5 μmol/L)-treated SW403 cells. d The corresponding semi-quantification data of (c). All values are shown as mean ± SD of three separate experiments, and significant values are indicated with asterisks (*P < 0.05, **P < 0.01 vs. control)