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Table 1 Comparison of 1H NMR data of fungal EDT with previously reported plant-derived 7-epi-10-deacetyltaxol and 10-deacetyltaxol chemical shifts (δ in ppm) and coupling constants (J, Hz)

From: Fungal 7-epi-10-deacetyltaxol produced by an endophytic Pestalotiopsis microspora induces apoptosis in human hepatocellular carcinoma cell line (HepG2)

H

Fungal 7-epi-10-deacetyltaxol

7-epi-10-deacetyltaxol (Jun et al. 2001)

10-deacetyltaxol (Mclaughlin et al. 1981)

7-epi-10-deacetyltaxol (Zhang et al. 2010)

δH

δH

J, Hz

δH

J, Hz

δH

J, Hz

2

5.78 (d)

5.726(d)

J = 7.44 Hz

    

5

4.88 (dd)

4.892(dd)

J = 4.09, 9.06 Hz

    

6

2.38, 2.28

2.32, 2.287

     

7

3.60 (s)

3.659 (s, broad)

     

7-OH

4.77(d)

    

4.73 (d)

J = 12.2 Hz)

0

5.65 (s)

5.415 (s)

     

10-OH

4.11

    

4.11(s, broad)

 

13

6.23 (t)

6.228 (t)

J = 8.55 Hz

    

14

2.29, 2.38

2.233, 2.36

     

16

1.09 (s)

1.072 (s)

     

17

1.18 (s)

1.183 (s)

     

20

4.42

4.411, 4.382 (AB)

J = 8.55 Hz

    

23

2.43 (s)

2.496 (s)

     

C-2 OBz

7.59 (m), 8.18 (d)

  

7.53 (m), 8.18 (d)

J = 2,8 Hz

  

OAc

2. 52 (s)

  

2.51 (s)

   

2’

4.78 (s, broad)

4.78 (s)

     

3’

5.78 (d)

5.796 (d)

J = 5.80, 9.00 Hz

    

3′-Ph

7.46 (m)

  

7.40 (m)

J = 9 Hz

  

3′-NBz

7.44 (m)

  

7.43 (m)

J = 2, 6 Hz

  

m/p

7.34 (s)

    

7.37/7.36

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