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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro

Fig. 4

Effect of CLSE on signaling pathways under hypoxic conditions. a. CLSE-treated HCT116 cells under hypoxic conditions for 72 h were analyzed by western blot analysis. ERK1/2 and AKT phosphorylation were downregulated by CLSE treatment under hypoxic conditions. b, c. After cells were treated with CLSE (1 mg/mL), PMA (5 ng/mL) or SC79 (5 μg/mL) for 8 h under hypoxic conditions, signaling proteins were examined through western blot analysis. d. Representative scratch-wound images showing the effect of PMA and SC79 on the healing ability of HCT116 cells (magnification: 40×). After cells were treated with CLSE (1 mg/mL), PMA (10 ng/mL), or SC79 (2.5 μg/mL) for 24 h under hypoxic conditions, migrating cells were analyzed using ImageJ software. e. Percentage of HCT116 cells that migrated to the wound area following combined treatment with CLSE and PMA or SC79. ** p < 0.001 versus normoxia control group. ## p < 0.01 versus hypoxia-only group. † p < 0.05, †† p < 0.01 versus hypoxia and CLSE treated group. Data are mean ± SD (n = 3). N, Normoxia; H, hypoxia; CLSE, Coix lacryma-jobi var. ma-yuen Stapf sprout extract; ERK, extracellular signal-regulated kinase; AKT, protein kinase B; PMA, phorbol 12-myristate 13-acetate; SD, standard deviation

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