Fig. 4

WERC suppresses osteoclast differentiation. BMMs were cultured in differentiation medium (DM) containing M-CSF and RANKL for 4 days with vehicle or WERC (10, 20, 40, 80, and 160 μg/mL). The cells cultured in DM were stained for (a) TRAP staining and were determined by the (b) TRAP activity assay on day 4 (100 × magnification). (c) TRAP-positive MNCs were counted on day 4. (d) The cells were cultured with WERC and M-CSF to examine cell viability on day 2. (e) The cells were cultured in DM in between additions of WERC (80 μg/mL) at the indicated days, and the osteoclast numbers were counted on day 4. *p < 0.05, **p < 0.01, ***p < 0.001