Fig. 1

Scheme of the experimental procedure. To induce AD-like inflammation 1% DNCB was topically applied to the dorsal back skin of female skh-1 hairless mice for 1 week once a day and boosted with 0.5% DNCB every alternate day for 3 weeks. After 3 weeks of boosting and bathing treatment, one more week of only bathing treatment was followed. Mice were either treated with Negative control group (NeC): only DNCB+ DW bathing, Positive control group (PC): DNCB +0.1% tacrolimus ointment + DW bathing; 100% pure high concentration mineral water (PHMW) group (PHMW): DNCB + PHMW bathing; 10% diluted high concentration mineral water (DHMW) group (DHMW): DNCB + DHMW bathing. On day 35, mice were sacrificed and blood samples were collected for further analysis