Fig. 5

Effects of SOCG treatment on the neurite outgrowth of DRG neurons. a-c. DRG at lumbar 4 and 5 were prepared from animals from 4 experimental groups. Neuritic processes of cultured DRG neurons were visualized by NF-200 staining (in green) and quantified (a). Representative images of DRG neurons for 4 experimental groups (b), and those from CRS + SOCG300 group (c). d-e. DRG neurons, which were prepared from the animal given sciatic nerve preinjury (SNI), were used for immunofluorescence staining with anti-NF-200 and anti-5-HT1AR antibodies. *P < 0.05; **P < 0.01 (One-way ANOVA, n = 4). All scale bars in (b), (c) and (e): 100 μm