Table 1 Real-time PCR primer sequences
From: Effects of Mollugo pentaphylla extract on monosodium urate crystal-induced gouty arthritis in mice
Gene | Primer sequence | |
|---|---|---|
IL-1β | Forward | 5′-TCTATACCTGTCCTGTGTAATGAAAG-3′ |
Reverse | 5′-GGCTTGTGCTCTGCTTGTGAG-3′ | |
TNF-α | Forward | 5′-GTGGAACTGGCAGAAGAG-3′ |
Reverse | 5′-CCATAGAACTGATGAGAGG-3′ | |
NF-κB | Forward | 5′-CTCACCGGCCTCATCCACAT-3′ |
Reverse | 5′-TGGCTAATGGCTTGCTCCAG-3′ | |
NALP3 | Forward | 5′-TGCTCTTCACTGCTATCAAGCCCT-3′ |
Reverse | 5′-ACAAGCCTTTGCTCCAGACCCTAT-3′ | |
ASC | Forward | 5′-CAGAGTACAGCCAGAACAGGACAC-3′ |
Reverse | 5′-GTGGTCTCTGCACGAACTGCCTG-3′ | |
Caspase-1 | Forward | 5′-TCCGCGGTTGAATCCTTTTCAGA-3′ |
Reverse | 5′-ACCACAATTGCTGTGTGTGCGCA-3′ | |
GAPDH | Forward | 5′-AGAAGGTGGTGAAGCAGGCATC-3′ |
Reverse | 5′-CGAAGGTGGAAGAGTGGGAGTTG-3′ |