Fig. 3

DKC-E70 mediates cyclin D1 degradation. a HCT116 cells were pretreated with MG132 at the indicated concentration for 2 h, and then co-treated with DKC-E70 (50 μg/ml) for 10 h. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1. b HCT116 ells were pretreated with DMSO or DKC-E70 (50 μg/ml), and then co-treated with 10 μg/ml of cycloheximide (CHX) for the indicated times. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1.Actin was used as internal control for Western blot analysis. *P < 0.05 compared to cell without DKC-E70 treatment