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Table 4 Cytotoxicity results of T cordifolia on HeLa cell line. Cytotoxicity results of T cordifolia by SRB assay on HeLa (Human cervical carcinoma)

From: Assessment of free radical scavenging and anti-proliferative activities of Tinospora cordifolia Miers (Willd)

S.No

Concentration (μg/mL)

% Cell Death

TCE

TCP

TCD

TCB

TCA

Doxorubicin

1

0.01

–

–

–

–

–

44.17 ± 0.38**

2

0.1

–

–

–

–

–

47.40 ± 0.17**

3

1

–

–

–

–

–

69.53 ± 0.02**

4

10

–

–

–

–

–

83.45 ± 0.18**

5

25

44.58 ± 0.19*

27.04 ± 0.95*

58.01 ± 0.11**

4.42 ± 3.68

10.64 ± 4.20

–

6

50

55.92 ± 0.71*

49.62 ± 0.35

59.46 ± 0.04**

27.50 ± 0.18

19.70 ± 2.62

–

7

100

69.84 ± 0.24**

58.60 ± 0.17*

76.68 ± 0.21**

42.10 ± 3.02*

33.42 ± 4.00

–

8

200

82.50 ± 0.83**

75.47 ± 0.12**

83.72 ± 0.19**

68.21 ± 2.45*

80.02 ± 0.38**

–

9

IC50

87.93 ± 0.85b

139.54 ± 1.54c

48.91 ± 0.33a,b

283.91 ± 2.93

377.39 ± 1.09

1.85 ± 0.02a

  1. Results were expressed as Mean ± SEM (n = 3) and analysed using one-way ANOVA followed by Tukey’s post hoc test of significance [where different alphabets denote significant difference (p < 0.05)]. Statistical comparison between the mean % viability of each extract and its negative control was performed using one-way ANOVA followed by Dunnett’s post hoc test of significance wherein *p < 0.05 and **p < 0.01 were considered to be statistically significant and very statistically significant compared to negative control. T cordifolia extracts were tested at concentrations of 200, 100, 50, 25 μg/mL and Doxorubicin was tested at concentrations of 0.01, 0.1, 1 and 10 μg/mL