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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Oral administration of taheebo (Tabebuia avellanedae Lorentz ex Griseb.) water extract prevents DSS-induced colitis in mice by up-regulating type II T helper immune responses

Fig. 5

TWE attenuates inflammatory responses in experimental colitis. a-d Mononuclear cells were collected 10 days after 3% DSS treatment in the presence or absence of TWE. a, b Intracellular IL12p40, IL6, TNFα, and IL10 productions were measured in the DCs (a) and macrophages (b) from the spleen and MLN using flow cytometry. The mean values ± SD (n = 5, * P < 0.05, ** P < 0.01) are shown. c Intracellular IFNγ, IL17, and IL4 productions were measured in the CD4+ T cells (CD3+CD4+) from the spleen and MLN using flow cytometry. The mean values ± SD (n = 5, * P < 0.05, ** P < 0.01) are shown. d Intracellular Foxp3 expression was measured in the Treg cells (CD3+CD4+CD25+) from the spleen and MLN using flow cytometry. In addition, the expression level of CTLA4 in Treg cells from the spleen and MLN (CD4+CD25+Foxp3+) was analyzed by flow cytometry. The graphs represent the MFI. The mean values ± SD (n = 5, * P < 0.05) are shown

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