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Table 2 Cytotoxicity and protein kinase inhibition of different solvent extracts of A. bracteosa

From: Polarity based characterization of biologically active extracts of Ajuga bracteosa Wall. ex Benth. and RP-HPLC analysis

Extracts Brine shrimp cytotoxicity THP-1 cytotoxicity Hep G2 cytotoxicity Protein kinase inhibition
% mortality LC50 (μg/ml) % inhibition IC50(μg/ml) % inhibition IC50 (μg/ml) Diameter (mm) at 100 μg/disc MIC (μg/disc)
200 μg/ml 20 μg/ml 20 μg/ml Clear zone Bald zone
Nh 63.33 ± 5.77bc 91.40 ± 0.23 31.5 ± 1.5 > 20 61.14 ± 0.70c 8.95 ± 0.20 10.00 ± 1.0 16.00 ± 1.0e 12.5
Nh-Ea 73.33 ± 5.77b 112.37 ± 1.20 45.57 ± 1.00c > 20 38.43 ± 0.78 > 20 6.67 ± 0.58 9.67 ± 0.58 100
Nh-E 53.33 ± 5.77cd 153.33 ± 1.12 35.87 ± 0.85 > 20 57.37 ± 0.67d 19.10 ± 0.50 6.67 ± 0.58 15.00 ± 1.0e 25
C 93.33 ± 5.77a 85.40 ± 0.26 26.67 ± 0.91 > 20 60.50 ± 0.50c 9.50 ± 0.28 6.67 ± 0.58 10.67 ± 0.58fg 50
Ea 43.33 ± 5.77 > 200 14.17 ± 1.35 > 20 61.53 ± 0.35c 8.63 ± 0.30 6.67 ± 0.58 9.33 ± 0.58 100
C-M 53.33 ± 5.77cd > 200 18.27 ± 2.91 > 20 20.60 ± 0.30 > 20 9.00 ± 1.0 12.00 ± 1.0f 100
Ea-M 63.33 ± 5.77bc 137.37 ± 0.55 19.63 ± 2.87 > 20 31.45 ± 0.15 > 20 9.00 ± 1.0 30.00 ± 1.0b 12.5
A 63.33 ± 5.77bc 123.83 ± 0.95 33.13 ± 1.70 > 20 54.71 ± 0.25e 19.80 ± 0.20 12.00 ± 1.0f 100
M 93.33 ± 5.77a 110 ± 0.75 45.37 ± 1.56c > 20 56.63 ± 0.20d 19.76 ± 0.20 28 ± 1.0 40.00 ± 1.0a 12.5
A-M 63.33 ± 5.77bc 123.5 ± 0.92 77.63 ± 1.33b 4.9 ± 0.20 25.90 ± 0.19 > 20 15.00 ± 1.0 25.0 ± 1.0c 50
A-E 93.33 ± 5.77a 123.4 ± 0.89 48.27 ± 3.00c > 20 49.80 ± 0.67 > 20 6.67 ± 0.58 12.0 ± 1.0f 100
E 96.67 ± 5.77a 46.91 ± 0.19 20.8 ± 1.90 > 20 37.40 ± 0.30 > 20 6.33 ± 0.58 7.67 ± 0.58
A-Dw 93.33 ± 5.77a 52.63 ± 1.11 47.43 ± 2.16c > 20 29.60 ± 0.80 > 20 7.00 ± 1.0 15.00 ± 1.0e 50
M-Dw 93.33 ± 5.77a 64.93 ± 1.40 30.47 ± 1.95 > 20 63.07 ± 0.40b 8.68 ± 0.25 12.00 ± 1.0f 100
Dw 33.33 ± 5.77 > 200 36.7 ± 1.15 > 20 46.83 ± 0.25 > 20 7.00 ± 1.0
Doxorubicin 96.67 ± 5.77a 5.63 ± 0.25    98.37 ± 0.15a 5.30 ± 0.20    
5-Florouracil    99.2 ± 0.92a 5.13 ± 0.13      
Vincristine    99.33 ± 0.83a 8.24 ± 0.12      
Surfactin         20 ± 1.0d  
DMSO      
  1. Values are presented as mean ± Standard deviation (n = 3). The values with different superscript (a-g) letters show significantly (P < 0.05) different means. --:No activity